Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase.
Identifieur interne : 001895 ( Main/Exploration ); précédent : 001894; suivant : 001896Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase.
Auteurs : Yuanheng Cai [États-Unis] ; Kewei Zhang [États-Unis] ; Hoon Kim [États-Unis] ; Guichuan Hou [États-Unis] ; Xuebin Zhang [États-Unis] ; Huijun Yang [États-Unis] ; Huan Feng [États-Unis] ; Lisa Miller [États-Unis] ; John Ralph [États-Unis] ; Chang-Jun Liu [États-Unis]Source :
- Nature communications [ 2041-1723 ] ; 2016.
Descripteurs français
- KwdFr :
- Biocarburants (MeSH), Biomasse (MeSH), Fermentation (MeSH), Génie génétique (MeSH), Lignine (métabolisme), Methyltransferases (composition chimique), Methyltransferases (génétique), Paroi cellulaire (métabolisme), Phénols (métabolisme), Polyosides (métabolisme), Populus (anatomie et histologie), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Végétaux génétiquement modifiés (MeSH), Éthanol (métabolisme).
- MESH :
- anatomie et histologie : Populus.
- composition chimique : Methyltransferases.
- croissance et développement : Populus.
- enzymologie : Populus.
- génétique : Methyltransferases, Populus.
- métabolisme : Lignine, Paroi cellulaire, Phénols, Polyosides, Éthanol.
- Biocarburants, Biomasse, Fermentation, Génie génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Biofuels (MeSH), Biomass (MeSH), Cell Wall (metabolism), Ethanol (metabolism), Fermentation (MeSH), Genetic Engineering (MeSH), Lignin (metabolism), Methyltransferases (chemistry), Methyltransferases (genetics), Phenols (metabolism), Plants, Genetically Modified (MeSH), Polysaccharides (metabolism), Populus (anatomy & histology), Populus (enzymology), Populus (genetics), Populus (growth & development).
- MESH :
- chemical , chemistry : Methyltransferases.
- chemical , genetics : Methyltransferases.
- chemical , metabolism : Ethanol, Lignin, Phenols, Polysaccharides.
- chemical : Biofuels.
- anatomy & histology : Populus.
- enzymology : Populus.
- genetics : Populus.
- growth & development : Populus.
- metabolism : Cell Wall.
- Biomass, Fermentation, Genetic Engineering, Plants, Genetically Modified.
Abstract
Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens' lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.
DOI: 10.1038/ncomms11989
PubMed: 27349324
PubMed Central: PMC4931242
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Kewei" sort="Zhang, Kewei" uniqKey="Zhang K" first="Kewei" last="Zhang">Kewei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726</wicri:regionArea><wicri:noRegion>Wisconsin 53726</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Guichuan" sort="Hou, Guichuan" uniqKey="Hou G" first="Guichuan" last="Hou">Guichuan Hou</name><affiliation wicri:level="1"><nlm:affiliation>Dewel Microscopy Facility, Appalachian State University, Boone, North Carolina 28608-2027, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Dewel Microscopy Facility, Appalachian State University, Boone, North Carolina 28608-2027</wicri:regionArea><wicri:noRegion>North Carolina 28608-2027</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xuebin" sort="Zhang, Xuebin" uniqKey="Zhang X" first="Xuebin" last="Zhang">Xuebin Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Huijun" sort="Yang, Huijun" uniqKey="Yang H" first="Huijun" last="Yang">Huijun Yang</name><affiliation wicri:level="1"><nlm:affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author><author><name sortKey="Feng, Huan" sort="Feng, Huan" uniqKey="Feng H" first="Huan" last="Feng">Huan Feng</name><affiliation wicri:level="1"><nlm:affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Earth and Environmental Studies, Montclair State University, Montclair, New Jersey 07043, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Earth and Environmental Studies, Montclair State University, Montclair, New Jersey 07043</wicri:regionArea><wicri:noRegion>New Jersey 07043</wicri:noRegion></affiliation></author><author><name sortKey="Miller, Lisa" sort="Miller, Lisa" uniqKey="Miller L" first="Lisa" last="Miller">Lisa Miller</name><affiliation wicri:level="1"><nlm:affiliation>National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726</wicri:regionArea><wicri:noRegion>Wisconsin 53726</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Chang Jun" sort="Liu, Chang Jun" uniqKey="Liu C" first="Chang-Jun" last="Liu">Chang-Jun Liu</name><affiliation wicri:level="1"><nlm:affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biology Department, Brookhaven National Laboratory, Upton, New York 11973</wicri:regionArea><wicri:noRegion>New York 11973</wicri:noRegion></affiliation></author></analytic><series><title level="j">Nature communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>Cell Wall (metabolism)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Genetic Engineering (MeSH)</term><term>Lignin (metabolism)</term><term>Methyltransferases (chemistry)</term><term>Methyltransferases (genetics)</term><term>Phenols (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Polysaccharides (metabolism)</term><term>Populus (anatomy & histology)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Fermentation (MeSH)</term><term>Génie génétique (MeSH)</term><term>Lignine (métabolisme)</term><term>Methyltransferases (composition chimique)</term><term>Methyltransferases (génétique)</term><term>Paroi cellulaire (métabolisme)</term><term>Phénols (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Populus (anatomie et histologie)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Methyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Methyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term><term>Lignin</term><term>Phenols</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Methyltransferases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Methyltransferases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Paroi cellulaire</term><term>Phénols</term><term>Polyosides</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Fermentation</term><term>Genetic Engineering</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biocarburants</term><term>Biomasse</term><term>Fermentation</term><term>Génie génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens' lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27349324</PMID><DateCompleted><Year>2018</Year><Month>08</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>04</Month><Day>24</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><PubDate><Year>2016</Year><Month>06</Month><Day>28</Day></PubDate></JournalIssue><Title>Nature communications</Title><ISOAbbreviation>Nat Commun</ISOAbbreviation></Journal><ArticleTitle>Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase.</ArticleTitle><Pagination><MedlinePgn>11989</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/ncomms11989</ELocationID><Abstract><AbstractText>Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens' lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Yuanheng</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Kewei</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hoon</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Guichuan</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Dewel Microscopy Facility, Appalachian State University, Boone, North Carolina 28608-2027, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xuebin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Huijun</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Huan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Earth and Environmental Studies, Montclair State University, Montclair, New Jersey 07043, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miller</LastName><ForeName>Lisa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, and the DOE Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Chang-Jun</ForeName><Initials>CJ</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6189-8756</Identifier><AffiliationInfo><Affiliation>Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nat Commun</MedlineTA><NlmUniqueID>101528555</NlmUniqueID><ISSNLinking>2041-1723</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="Y">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName 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IdType="pubmed">9439593</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Cai, Yuanheng" sort="Cai, Yuanheng" uniqKey="Cai Y" first="Yuanheng" last="Cai">Yuanheng Cai</name></noRegion><name sortKey="Feng, Huan" sort="Feng, Huan" uniqKey="Feng H" first="Huan" last="Feng">Huan Feng</name><name sortKey="Feng, Huan" sort="Feng, Huan" uniqKey="Feng H" first="Huan" last="Feng">Huan Feng</name><name sortKey="Hou, Guichuan" sort="Hou, Guichuan" uniqKey="Hou G" first="Guichuan" last="Hou">Guichuan Hou</name><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name><name sortKey="Liu, Chang Jun" sort="Liu, Chang Jun" uniqKey="Liu C" first="Chang-Jun" last="Liu">Chang-Jun Liu</name><name sortKey="Miller, Lisa" sort="Miller, Lisa" uniqKey="Miller L" first="Lisa" last="Miller">Lisa Miller</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Yang, Huijun" sort="Yang, Huijun" uniqKey="Yang H" first="Huijun" last="Yang">Huijun Yang</name><name sortKey="Zhang, Kewei" sort="Zhang, Kewei" uniqKey="Zhang K" first="Kewei" last="Zhang">Kewei Zhang</name><name sortKey="Zhang, Xuebin" sort="Zhang, Xuebin" uniqKey="Zhang X" first="Xuebin" last="Zhang">Xuebin Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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